Get Comprehensive Guide to Double Marker Test in India – Reasons, Risks, Interpretation, Cost and Booking Details

Comprehensive guide to Double Marker Test in India / Double Marker Test Cost in India

Here is a complete guide containing all the information about Double Marker Test by The details are covered under following topics:

  1. What is a Double Marker Test?
  2. Why is it done?
  3. What is the cost of Double Marker Test?
  4. The science behind the Double Marker Test
  5. How is Double Marker Test done?
  6. What are the risks and side-effects of Double Marker Test?
  7. How to interpret the result of the test?
  8. Accuracy of Double Marker Test.
  9. How to book the Double Marker Test in India ?

To quickly book a Double Marker test, give us a call or WhatsApp at 08882668822. If you will like a call back, please fill out the form given below.


What is a Double Marker Test?

Double Marker test is one of pre-natal screening tests done in the first trimester of the pregnancy. It is done to assess the chances of your foetus having chromosomal abnormality. It helps you detect if your child to be born, will have birth defects like Down’s Syndrome or trisomy 18 which are basically chromosomal disorders. These disorders can lead to serious mental defects in a child and sometimes may result in stillbirth.

Cost of the Double Marker Test in India

Double Marker Test Cost varies between ₹ 1320 to ₹ 2880 in India. This cost variation depends on your city and the chosen lab. This link contains the cost of double marker test in different cities of India. For some metros, the prices are given in the table below.

Double Marker Test Labsadvisor min. Cost of Double Marker test Market Price of Double Marker Test
Cost of Double Marker Test in Delhi ₹ 1320 ₹ 2800
Cost of Double Marker Test in Noida ₹ 1450 ₹ 2800
Cost of Double Marker Test in Gurgaon ₹ 1320 ₹ 3000
Cost of Double Marker Test in Mumbai ₹ 1350 ₹ 1930
Cost of Double Marker Test in Chennai ₹ 2880 ₹ 1930
Cost of Double Marker Test in Hyderabad ₹ 1870 ₹ 2200
Cost of Double Marker Test in Bangalore ₹ 2125 ₹ 1930
Cost of Double Marker Test in Ghaziabad ₹ 1450 ₹ 2500
Cost of Double Marker Test in Faridabad ₹ 1600 ₹ 2800

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 Why is Double Marker Test done?

Double marker test is significant as it helps you be assured that a healthy baby is growing inside. Double Marker test is done to evaluate the risk of your baby having down’s syndrome or trisomy 18.

Down’s Syndrome: Down’s syndrome is also known as Trisomy 21. Normally foetal DNA will have 23 pairs of chromosomes, 23 from the mother and the other 23 from the father. But sometimes instead of 46 chromosomes, the DNA carries an extra copy of chromosome or has a missing or structurally altered chromosome leading to genetic disorders. Down’s syndrome is caused when the foetal DNA contains extra copy of chromosome 21. People with Down’s syndrome have following characteristics:

  • Abnormal facial features, slant eyes and small ears.
  • Delayed development and intellectual impairment.
  • Hearing and vision problem
  • Seizures
  • Increase risk of pulmonary hypertension and Heart disease.
  • Low muscle tone
  • Thyroid problems

The likelihood of a baby born with down’s syndrome increase with mother’s age. Higher the age of the mother, higher are the chances of her baby having down’s syndrome. So expectant mothers of age more than 35 should definitely opt for double marker test.

Trisomy 18: Trisomy 18 is also known as Edwards syndrome. An extra copy of chromosome 18 in foetal DNA causes this serious disorder. Most of the babies born with Edwards syndrome die in their infancy. Around one in twelve babies live beyond one year but with severe mental disabilities. There is no cure of Edwards syndrome and symptoms are difficult to manage. Children with Edwards syndrome have to be regularly monitored for infections and heart problems.

Like Down’s syndrome, the chances of baby to be born with Edwards syndrome increases with mothers age. So older mothers should go for Double marker screening to identify the risk and hence make a choice to continue the pregnancy.

The test is often most recommended for women who:

  • Are older than 35
  • have a family history of birth defects
  • have diabetes
  • have been exposed to high levels of radiation
  • had a viral infection during pregnancy

Mothers-to-be who do not have above issues may still choose to have double-marker test to assess the risk and hence e prepared accordingly.

The Science behind the test

Double marker test is a simple maternal blood test. It checks for the levels of two specific substances in the blood. These two blood markers are Free Beta HCG and PAPP-A.

Free Beta HCG: Free Beta Human Chorionic Gonadotropin is a hormone released by the placenta after embryo implantation. This hormone helps in the development of your baby and keep the pregnancy going. The levels of HCG go up fast during the first trimester. The levels are highest around the 14th week of last menstrual period. Then the levels go down gradually.The amount of HCG in your blood can give information about your pregnancy and health of your baby. After delivery no HCG is found. More HCG is released in case of multiple pregnancy as compared to single pregnancy. Low amount of HCG is released if the embryo is located other than the uterus i.e. ectopic pregnancy. If you are pregnant and   your HCG levels are going down abnormally, a miscarriage is very likely.

PAPP-A: Pregnancy Associated plasma protein A is a protein produced by the embryo and the placenta.Low levels of PAPP-A can be associated with Down’s syndrome.Low levels may also be associated with low birth weight and early delivery. Hence an extra scan should be done to check the growth of baby if low PAPP-A is found. Increased level of PAPP-A indicates increased risk of trisomy 21. Reduced levels of PAPP-A are associated with an increased chances of trisomy 18. If first trimester PAPP-A level is less than 0.4 MOM then there is a high risk of adverse obstetrical outcomes like intra-uterine growth restriction, premature delivery and still birth.

Test Down’s Syndrome Trisomy 18
HCG High Low
PAPP-A Low Low

How is the Double Marker test done?

Double Marker is a simple maternal serum test.You don’t need any special preparations for the test. You can eat or drink normally before the test.

An elastic band will be wrapped around your upper arm.The site will be cleaned by alcohol. A needle is injected in a vein of your arm. A tube is attached to the needle to fill it with blood. After sufficient amount of blood is drawn, the needle is withdrawn and pressure is put at the site. A small bandage might be put to stop bleeding. The whole procedure will take few minutes.

Book Double Marker Test in Delhi at Affordable Prices – Double Marker Test in India

What are the Risks and side-effects of Double Marker test?

As double marker test is a blood test of the mother so no harm is caused to the foetus.So there is no risk of miscarriage or other pregnancy related complications.

You may get small bruise at the site of the needle injection.

In rare cases, the vein may become swollen. A warm compress can be used at the site several times a day.

If you are suffering from bleeding or clotting problem or if you take blood thinning medicine like aspirin, tell your doctor before blood sample is taken.

How to interpret the result of the test?

A mathematical calculation involving the levels of these substances (HCG and PAPP-A) and considerations of maternal age, family history, body weight, race, and diabetic status are used to evaluate a numeric risk for Down syndrome and for Edwards syndrome, in the foetus. This risk is then compared with an established cut-off. If the risk is more than the cut-off value, then it is considered a positive screening test.

A positive screen test means the chances of the foetus having one of the chromosomal disorder is higher than usual. Your doctor will then recommend further diagnostic tests like CVS or amniocentesis. CVS is performed in the first trimester from 10 to 13 weeks of gestation, whereas amniocentesis can be performed starting at 15 weeks of gestation.

You may be advised a new blood screening (NIPT or Harmony testing) that evaluates the foetal DNA in a woman’s blood and can give doctors an indication about chromosomal abnormalities.

A positive screen test does not necessarily mean that the baby will be born with birth defect.

If the risk is less than the cut off risk, then the result is screen negative. You do not need to go for further diagnostic test.

Accuracy of the test

The accuracy of the test varies from lab to lab. This test correctly detects down’s syndrome in 69 out of 100 cases. When done in conjunction with ultrasound the accuracy increases to 79%. This means that the test misses to detect the condition in 21 cases out of hundred cases. There is also a chance of getting false positive i.e. result showing high risk of defects while the baby is totally normal.  This can lead to unnecessary stress and further testing. The doctor or genetic counselor will advise you after taking other factors like hereditary, age, race and diabetics into consideration.

How to book the Double Marker test in India ?

To book a test in a diagnostic lab near you just give us a call or send WhatsApp message at 08882668822. You can also download our Android App ‘LabsAdvisor’ from Play store and book the test. If you want a call back, fill out the form given at the start of the article.

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