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HPLC / Thalassemia Test Cost

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HPLC Test Price₹ 480
Thalassemia Profile Test Cost₹ 1,200

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Lowest HPLC Test Cost and Thalassemia Test Cost in Delhi, Gurgaon, Bangalore, Mumbai, Chennai, Hyderabad, etc.
HPLC Test & Thalassemia Test Price.

Frequently Asked Questions About Thalaseemia or HPLC

What is Thalassemia?

Thalassemia is a genetic disease of blood disorder causing increased destruction of red blood cells. As a result, the patient has low hemoglobin and less red blood cell count which leads to anemia.

What are the Types of Thalassemia?

Normal hemoglobin also called as hemoglobin has four protein chains, two Alpha globin and two beta globin. Deficiency in these protein chains lead to two types of Thalassemia:

Alpha Thalassemia: Alpha Thalassemia occurs when the body can’t make a protein called alpha globin. To make alpha globin, one needs to have four genes, two from each parent. alpha Thalassemia minor is caused when one or two genes are absent. If three genes are absent then a disease called Hemoglobin H is caused. Absence of all the four gene causes Hydrops fetalis which is extremely severe and occurs before birth. Survival is very rare.

Beta thalassemia: Beta thalassemia occurs when the body can’t produce a protein called beta globin. Two genes including one from each parent, are inherited to make beta globin. Absence of one gene cause thalassemia minor while absence of both the genes will lead to the serious form of disease called as Beta thalassemia major.

According to the severity of the disease, thalassemia can be categorized as:

Thalassemia Major: A child suffering from severe anemia starts depending on blood transfusions throughout its whole life as both the genes producing normal hemoglobin are damaged..

Thalassemia intermedia: It is a less severe form. It develops because of alterations in both beta globin genes. People with thalassemia intermedia do not need blood transfusions.

Thalassemia Minor: It is a mild condition. Usually the patients are asymptomatic or have mild anemia. Thalassemia minor patients are also called as carriers as they carry a faulty gene and are more likely to give birth to thalassemia major babies.

Different types of Thalassemia.
Types of Thalassemia.

What are the Causes of Thalassemia?

Thalassemia is inherited and at least one of the parents must be a carrier of the disease. If both the parents are carriers, then there is one in four chance of their baby to have Thalassemia major. It is not possible to contract Thalassemia from environment and it is not an infectious disease.

What are the Signs and Symptoms of Thalassemia?

Patients with beta Thalassemia intermedia have mild to moderate anemia.

Patients with Beta Thalassemia have severe anemia. The signs and symptoms of disease usually occur within the first 2 years of life. They may include the following health problems

  • Bone deformities, especially in the face
  • Dark urine
  • Excessive tiredness and fatigue
  • Yellow or pale skin
  • Poor appetite, poor growth, tiredness.
  • An enlarged spleen and liver.
  • Bone problems (especially with bones in the face).

How is Thalassemia Diagnosed?

To diagnose the disease, generally HPLC in conjunction with CBC is done. The CBC readings may indicate Thalassemia which is then confirmed by HPLC test.

To know more about CBC Test, click here

Applications of HPLC or Thalassemia Test.
Uses of Thalassemia test.

What is a HPLC Test?

High-Performance Liquid Chromatography is the full form of HPLC. It is a technique used to split each component from a mixture. It separates the hemoglobin types present in a sample of blood. HPLC Test is the most accurate diagnosis for screening of beta Thalassemia carriers today.

HPLC is a simple blood test to measure ‘Hb A2′ in blood by Hemoglobin Chromatography. The test does not require any special preparation and can be done at any time of the day.As it is a simple blood test, no risk is involved.

What can I know from the HPLC Test result?

If the HbA2 is less than 3.8 then you are not a Thalassemia minor.

If the HbA2 value is more than 3.8, you may be a confirmed case of Thalassemia Minor. Following precautions may be required

  • If you are unmarried, avoid marrying Thalassemia Minor.
  • If you are already married, get your spouse tested for Thalassemia Minor condition.
  • If your spouse is not a Thalassemia Minor, no precaution is required.

What is HPLC Test in Pregnancy?

Some types of Thalassemia may affect your fertility and make difficult to get conceive. If both you and your spouse are carrying the Thalassemia trait, consult your doctor for genetic counseling before planning your baby and get diagnosis done to rule out Thalassemia major in the unborn child so that the pregnancy can be terminated if you want to.

Who Should be Screened for Thalassemia?

  • Pre-marital youth (18-25 years of age).
  • Newly married couples who are planning to start their family
  • Pregnant women in their first trimester.
  • Parents and extended family of Thalassemia major children or individuals belonging to the high-risk communities.
  • Anybody who has persistent and unexplainable anemia.
  • Any individual with a raised RBC count.
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